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h8 human cervical epithelial cell line  (ATCC)


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    ATCC h8 human cervical epithelial cell line
    H8 Human Cervical Epithelial Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 498 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/epithelial+cell+line+h8/Primary+Cervical+Epithelial+Cells/pmc13039669-43-7-24
    Average 96 stars, based on 498 article reviews
    h8 human cervical epithelial cell line - by Bioz Stars, 2026-10
    96/100 stars

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    other:

    Article Title: LncRNA PVT1 promotes cervical cancer progression by sponging miR-503 to upregulate ARL2 expression
    Article Snippet: Human normal immortalized cervical epithelial cell line (H8) was purchased from Institute of Preclinical Medicine, Peking Union Medical University (Beijing, China), and CC HeLa (CCL-2) and SIHA (HTB-35) cells were acquired from the American Type Culture Collection (ATCC, Rockville, MD, USA).

    Article Title: Histone Demethylase KDM3A Promotes Cervical Cancer Malignancy Through the ETS1 /KIF14/Hedgehog Axis
    Article Snippet: An immortalized cervical epithelial cell line (H8) was from Cell Resource Center, the Chinese Academy of Sciences (Shanghai, China), and CC cell lines (HT-8, CaSki, HeLa, and SiHa) were from American Type Culture Collection (ATCC, Manassas, VA, USA).

    Transfection:

    Article Title: <p>Histone Demethylase <em>KDM3A</em> Promotes Cervical Cancer Malignancy Through the <em>ETS1</em>/KIF14/Hedgehog Axis</p>
    Article Snippet: .. Cell Transfection An immortalized cervical epithelial cell line (H8) was from Cell Resource Center, the Chinese Academy of Sciences (Shanghai, China), and CC cell lines (HT-8, CaSki, HeLa, and SiHa) were from American Type Culture Collection (ATCC, Manassas, VA, USA). .. All cells were cultivated in 10% fetal bovine serum (FBS)supplemented DMEM containing 1% penicillin/streptomycin (all from Sigma-Aldrich Chemical Company, St Louis, MO, USA) at constant 37°C with 5% CO2.



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    Circ_0002762 is up-regulated in CSCC cells (A) GSE102686 was analysed to screen for circRNAs aberrantly expressed in CSCC, leading to the identification of circ_0081672 and circ_002762 as candidate circRnas. (B) Expression profiles of circ_0002762 and circ_0081672 in CSCC tissues and paired para-cancerous cervical tissues were obtained from GSE102686. (C) Rt-qPCR was performed to examine expression levels of circ_0002762 and circ_0081672 in the three CSCC cell lines and cervical epithelial cell line (H8), revealing that circ_0002762 is highly expressed in CSCC cells. (D) Schematic diagram was presented to illustrate the splicing pattern of circ_0002762 (312nt). (E) Following ActD treatment, Rt-qPCR analysis showed that the mRNA stability of circ_0002762 is stronger than that of CDK17 mRNA. (F) RNA extractions were treated with RNase R and remaining levels were quantified with Rt-qPCR, indicating a significant level of circ_0002762. ** p < 0.01; N.S.: no significance.

    Journal: RNA Biology

    Article Title: Circular RNA Circ_0002762 promotes cell migration and invasion in cervical squamous cell carcinoma via activating RelA/nuclear factor kappa B (Nf-kB) signalling pathway

    doi: 10.1080/15476286.2025.2478539

    Figure Lengend Snippet: Circ_0002762 is up-regulated in CSCC cells (A) GSE102686 was analysed to screen for circRNAs aberrantly expressed in CSCC, leading to the identification of circ_0081672 and circ_002762 as candidate circRnas. (B) Expression profiles of circ_0002762 and circ_0081672 in CSCC tissues and paired para-cancerous cervical tissues were obtained from GSE102686. (C) Rt-qPCR was performed to examine expression levels of circ_0002762 and circ_0081672 in the three CSCC cell lines and cervical epithelial cell line (H8), revealing that circ_0002762 is highly expressed in CSCC cells. (D) Schematic diagram was presented to illustrate the splicing pattern of circ_0002762 (312nt). (E) Following ActD treatment, Rt-qPCR analysis showed that the mRNA stability of circ_0002762 is stronger than that of CDK17 mRNA. (F) RNA extractions were treated with RNase R and remaining levels were quantified with Rt-qPCR, indicating a significant level of circ_0002762. ** p < 0.01; N.S.: no significance.

    Article Snippet: Cervical epithelial cell line H8 and three cervical cancer cell lines including HeLa, CaSki and C33A were procured from the American Type Culture Collection (ATCC).

    Techniques: Expressing, Quantitative RT-PCR

    ELK4 is highly expressed in HPV+ CC patients. (a) The mRNA expression of ELK4 in cervical tissues was compared with cervix cancer tissues by qRT-PCR. (b) Western blot assay showed the protein expression of ELK4 in cervical tissues and cervix cancer tissues. β-actin was normalized control. (c) ELK4 protein levels in human cervical epithelial immortalized cell line H8 and Cervix cancer cell lines (HeLa, CaSki, and SiHa) were detected by western blot. β-actin was normalized. The data are expressed as the mean ± SD. ***p < 0.001 vs normal tissues or cell line H8. SD, Standard deviation

    Journal: Balkan Medical Journal

    Article Title: ELK4 Promotes Cell Cycle Progression and Stem Cell-like Characteristics in HPV-associated Cervical Cancer by Regulating the FBXO22/PTEN Axis

    doi: 10.4274/balkanmedj.galenos.2023.2023-4-66

    Figure Lengend Snippet: ELK4 is highly expressed in HPV+ CC patients. (a) The mRNA expression of ELK4 in cervical tissues was compared with cervix cancer tissues by qRT-PCR. (b) Western blot assay showed the protein expression of ELK4 in cervical tissues and cervix cancer tissues. β-actin was normalized control. (c) ELK4 protein levels in human cervical epithelial immortalized cell line H8 and Cervix cancer cell lines (HeLa, CaSki, and SiHa) were detected by western blot. β-actin was normalized. The data are expressed as the mean ± SD. ***p < 0.001 vs normal tissues or cell line H8. SD, Standard deviation

    Article Snippet: The human cervical epithelial immortalized cell line H8 (PCS-480-011) and cervical carcinoma cell lines such as HeLa (HPV18, CRM-CCL-2), CaSki (HPV16, CRM-CRL-1550), and SiHa (HPV − , HTB-35) (USA) were obtained from the American Type Culture Collection.

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control, Standard Deviation